Table 2.
Ab513 Breadth of Binding to Diverse Genotypes Determined by Competition ELISA and SPR, with In Vitro Neutralization of Select Strains
| Serotype | EDIII strain | ELISA KD (nM) | Fold increase from 4E5A | SPR KD (nM) | EC50 (ng/ml)a |
|---|---|---|---|---|---|
| I | Hawaii/1944 | <0.1 | NDb | 0.041 | 67 ± 12 |
| Vietnam/2008 | <0.1 | ND | 0.039 | ||
| Malaysia/2005 | <0.1 | ND | 0.064 | ||
| Mexico/2007 | <0.1 | ND | 0.071 | ||
| II | New Guinea/1944 (NGC) | <0.1 | ND | 0.015 | 190 ± 55 |
| Singapore/2008 | <0.1 | ND | 0.024 | ||
| Peru/1995 | <0.1 | ND | 0.012 | ||
| Vietnam/2007 | <0.1 | ND | 0.014 | ||
| Venezuela/2007 | <0.1 | ND | 0.029 | ||
| IIII | Philippines/1956 (H87) | 1.24 | 12.8 | 1.0 | 97 ± 20 |
| Singapore/2009 | 0.35 | 18.3 | 0.6 | ||
| Nicaragua/2010 | 3.36 | 9.5 | 4.9 | ||
| Puerto Rico/1977 | 1.36 | 9.3 | 1.4 | ||
| Cambodia/2008 | 1.32 | 10.1 | 2.5 | ||
| IV | Mexico/1997 (BC287/97) | 4.32 | 21.8 | 3.8 | 79 ± 30 |
| Singapore/2010 | 8.43 | 14.4 | 6.1 | ||
| New Caledonia/2009 | 10.81 | 15.9 | 8.9 | ||
| Philippines/1956 (H241) | 113.10 | 40.7 | 118.5 | 2,300 ± 1,500 | |
| Brazil/2011 | 14.68 | 18.2 | 7.9 | ||
| Venezuela/2008 | 4.50 | 11.9 | 3.0 | ||
| Thailand/1997 | 611.60 | 6.6 | >300c |
a
Average ± SEM, from three independent experiments.
b
ND, not determined.
c
Affinity estimate based on curve.