Table 1.

Material properties of acellular hybrid scaffolds (n=5)

Molar ratio of norbornene succinimidyl ester reacted with gelatin amines	Norbornene Functionalization Efficiencya (%)	Calculated amount of norbornene on gelatin chainb (mM)	Thiol concentration (mM)	Swollen Shear Modulus G (kPa)	Crosslinking Densityc ρxL (mM)
0.25:1 Norbornene:gelatin amines	19 ± 5%	~1.1	0.6	<0.1	<0.18
0.5:1 Norbornene: gelatin amines	36 ± 5%	~2.2	0.6	0.2 ± 0.1	0.36 ± 0.04
1:1 Norbornene:gelatin amines	75 ± 6%	~4.5	0.6	0.5 ± 0.1	0.90 ± 0.05

^aNorbornene functionalization efficiency was calculated using $\{1-\frac{\left[\mathit{\text{amines}}\right]\mathit{\text{after}}\mathit{\text{modification}}}{\left[\mathit{\text{amines}}\right]\mathit{\text{before}}\mathit{\text{modification}}}\}\times 100\%$

^bNorbornene concentration per gelatin molecule was calculated by accounting for the amount of amines on type A gelatin (~21) and the norbornene functionalization efficiency for each condition assuming only norbornenes replaced the gelatin amines.

^cCrosslinking density was calculated from rubber elastic theory: ρ_xL= GQ^1/3(RT)⁻¹