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. 2016 Feb 18;11(2):e0149660. doi: 10.1371/journal.pone.0149660

Fig 5. Viable cells in proliferation after vitrification.

Fig 5

The CellTiter 96® AQueous One Solution Cell Proliferation Assay (COS, Promega Corporation, Mannheim, Germany) was used to determine viable cells in proliferation at one hour after thawing and at day one, two, and three. Figure 5 illustrates the absorption of viable cells in proliferation after vitrification. DMEM = DMEM Ham’s F-12 Gln+, 20% FBS, and 3M of one of the CPAs; K+TiP = K+TiP. 0.5M sucrose, 20% FBS, and 3M of one of the CPAs; DMSO = dimethyl sulfoxide; EG = ethylene glycol; PG = propylene glycol; GLY = glycerin; * = significant (p < 0.05); ** = medium significant (p < 0.01); au = absorbance units.