Table 1. Composition of K+TiP.
Cl- | 94 | mM |
H2PO4- | 1 | mM |
α-ketoglutarate | 2 | mM |
aspartate | 5 | mM |
Na+ | 21 | mM |
K+ | 131 | mM |
Mg2+ | 7 | mM |
Ca2+ | 0.02 | mM |
N-acetylcysteine | 27 | mM |
glycine | 9 | mM |
alanine | 5 | mM |
tryptophan | 2 | mM |
saccharose | 27 | mM |
glucose | 9 | mM |
Desferal | 0.075 | mM |
LK 614 | 0.015 | mM |
osmolarity | 338 | mosm/l |
pH | 6.93 |
K+TiP was prepared by supplementing 500 mL TiProtec (Dr. Franz Köhler Chemie GmbH, Bensheim, Germany) with 0.871g K2SO4 and 3.87g 56.3% w/w potassium lactate and was diluted with 50mL distilled water prior to use. Table 1 shows the final composition of K+TiP.