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. 2016 Feb 18;11(2):e0149660. doi: 10.1371/journal.pone.0149660

Table 1. Composition of K+TiP.

Cl- 94 mM
H2PO4- 1 mM
α-ketoglutarate 2 mM
aspartate 5 mM
Na+ 21 mM
K+ 131 mM
Mg2+ 7 mM
Ca2+ 0.02 mM
N-acetylcysteine 27 mM
glycine 9 mM
alanine 5 mM
tryptophan 2 mM
saccharose 27 mM
glucose 9 mM
Desferal 0.075 mM
LK 614 0.015 mM
osmolarity 338 mosm/l
pH 6.93

K+TiP was prepared by supplementing 500 mL TiProtec (Dr. Franz Köhler Chemie GmbH, Bensheim, Germany) with 0.871g K2SO4 and 3.87g 56.3% w/w potassium lactate and was diluted with 50mL distilled water prior to use. Table 1 shows the final composition of K+TiP.