Table 7. Infection and feeding source determination.
We used two methods to determine Trypanosoma cruzi infection in bugs: direct examination (Dir-Inf) and PCR. Ni, is the number of positive individuals; Nf, the total number of examined individuals. Parasite characterization (TCII/TCI) was determined by PCR (see Materials and Methods). The number of feeding sources detected is indicated and their putative affiliation (putative feeding source) using BLAST searches. The Id (%) represents the identity of the sequences obtained from vector guts with those in GenBank (GB), with the GB code in the last row. Feeding sources detected in vectors infected by T. cruzi via PCR are in bold.
| Pop | Dir-inf, Ni/Nt (%) | PCR-Inf, Ni/Nt (%) | TCII/TCI | Feeding sources detected (%) | Number of feeding sources | Putative feeding source | id % | Gb code | Gb species affiliation and popular name |
|---|---|---|---|---|---|---|---|---|---|
| A (Syl-c) | 24/29 (83) | 17/24 (70.8) | 17/0 | 8/24 (33) | 3 | Felis catus | 100 | AY509646 | Felis catus (domestic cat) |
| 4 | Kerodon rupestris | 96 | GU136722 | Kerodon rupestris (rock cavy) | |||||
| 1 | Proceratophrys sp. | 83 | KF214166 | Proceratophrys boiei (frog) | |||||
| C (Syl-c) | 18/19 (95) | 13/25 (52.0) | 11/2 | 20/25 (80) | 2 | Felis catus | 100 | AY509646 | Felis catus |
| 1 | Galea sp. | 88 | GU067492 | Galea spixii (Spix's yellow-toothed cavy) | |||||
| 15 | Keradon rupestris | 96 | GU136722 | Keradon rupestris | |||||
| 1 | Thrichomys apereoides | 98 | AY083338 | Thrichomys apereoides (spiny rat) | |||||
| 1 | Homo sapiens | 97 | KJ8556840 | Homo sapiens (human) | |||||
| F (Syl-d) | - | 5/19 (26.3) | 5/0 | 6/19 (31.6) | 3 | Galea spixii | 96 | GU067492 | Galea spixii |
| 2 | Capra hircus | 100 | FM205715 | Capra hircus (goat) | |||||
| 1 | Felis catus | 100 | AY509646 | Felis catus | |||||
| D (Per) | 0/25 (0) | 1/28 (3.6) | 1/0 | 11/28 (39.3) | 9 | Gallus gallus | 100 | DQ512917 | Gallus gallus (chicken) |
| 1 | Mabuya sp. | 79 | AF280276 | Mabuya spinalis (lizard) | |||||
| 1 | Proceratophrys sp. | 78 | KF214166 | Proceratophrys boiei | |||||
| H (Per) | - | 0/9 (0) | 0/0 | 8/9 (88.9) | 8 | Galea spixii | 96 | GU067492 | Galea spixii |
| B (Dom) | 0/17 (0) | 0/21 (0) | 0/0 | 4 | Capra hircus | 100 | FM205715 | Capra hircus | |
| 2 | Gallus gallus | 100 | DQ512917 | Gallus gallus | |||||
| 1 | Proceratophrys sp. | 83 | JN814534 | Proceratophrys boiei | |||||
| Total | 42/90 (46.5) | 36/126 (28.6) | 32/2 | 60/126 (47.6) | 60 |
Syl = sylvatic, Per = peridomiciliary and Dom = domiciliary, Syl-c = conserved sylvatic, Syl-d degraded sylvatic.