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. 2016 Feb 18;11(2):e0149489. doi: 10.1371/journal.pone.0149489

Fig 3. Optimization of temperature control affects tubular necrosis and neutrophil infiltration.

Fig 3

Male C57BL/6N mice, 6–8 weeks of age, underwent unilateral ischemia for 45 minutes and subsequent reperfusion for 24 hours using setup A, B and C, respectively. (A) Representative PAS-stained pictures of sham-operated and ischemic kidney sections are shown in 100-fold magnification. The necrotic area of the S3 segment [%] is presented as mean ± SEM, derived from n = 5. (B) Representative Ly-6B.2-stained pictures of sham-operated and ischemic kidney sections are shown in 20-fold magnification. Ly-6B.2-positive events are assessed from 50-fold magnified images and normalized to the entire kidney section area, expressed as mean ± SEM, derived from n = 5.