Skip to main content
. 2016 Feb 18;11(2):e0149489. doi: 10.1371/journal.pone.0149489

Fig 4. Optimization of temperature control affects mRNA expression levels of pro-inflammatory and kidney injury markers.

Fig 4

Male C57BL/6N mice, 6–8 weeks of age, underwent unilateral ischemia for 45 minutes and subsequent reperfusion for 24 hours using setup A, B and C, respectively. mRNA expression levels were assessed by reverse transcription and subsequent qRT-PCR for (A) Kim-1, (B) Ngal, (C) Cxcl-2 and (D) Il-6. Data are calculated as target gene expression normalized to the housekeeping gene 18s and presented as mean ± SEM, derived from n ≥ 5.