Fig 5. Heating chamber-based optimization of temperature control enables precise modulation of tubular injury and neutrophil infiltration at different ischemia times.

Male C57BL/6N mice, 6–8 weeks of age, underwent sham surgery or unilateral ischemia for 15, 25, 35 or 45 minutes and subsequent reperfusion for 24 hours using setup C. (A) Representative PAS-stained pictures of sham-operated and ischemic kidney sections are shown at 100-fold magnification. The necrotic area of the S3 segment [%] is presented as mean ± SEM, derived from n = 5. (B) Representative Ly-6B.2-stained pictures of sham-operated and ischemic kidney sections are shown at 20-fold magnification. Ly-6B.2-positive events are assessed from 50-fold magnified images and normalized to whole kidney section area, expressed as mean ± SEM, derived from n = 5.