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. 2016 Feb 18;11(2):e0149489. doi: 10.1371/journal.pone.0149489

Fig 6. Heating chamber-based optimization of temperature control enables precise modulation of kidney injury and inflammatory marker expression by changing ischemia time.

Fig 6

Male C57BL/6N mice, 6–8 weeks of age, underwent sham surgery or unilateral ischemia for 15, 25, 35 or 45 minutes ischemia and subsequent reperfusion for 24 hours using setup C. mRNA expression levels were assessed by reverse transcription and subsequent qRT-PCR for (A) Kim-1, (B) Ngal, (C) TNFα, (D) Il-6, (E) MCP-1 and (F) Cxcl-2. Data are calculated as target gene expression normalized to the housekeeping gene 18s and presented as mean ± SEM, derived from n ≥ 5.