Table 1. Primer pairs used in this study.
| Target | Accession no | Forward primer | Reverse primer | efficiency |
|---|---|---|---|---|
| 18s | NM 013693.3 | GCAATTATTCCCCATGAACG | AGGGCCTCACTAAACCATCC | 1.89 |
| Cxcl-2 | NM 009140.2 | CGGTCAAAAAGTTTGCCTTG | TCCAGGTCAGTTAGCCTTGC | 1.98 |
| Il-6 | NM 013541.1 | TGATGCACTTGCAGAAAACA | ACCAGAGGAAATTTTCAATAGGC | 1.97 |
| Kim-1 | NM 031168.1 | TCAGCTCGGGAATGCACAA | TGGTTGCCTTCCGTGTCTCT | 1.96 |
| Mcp-1 | NM 001166632.1 | CCTGCTGTTCACAGTTGCC | ATTGGGATCATCTTGCTGGT | 2.01 |
| Ngal | NM 011333.3 | AATGTCACCTCCATCCTGGT | ATTTCCCAGAGTGAACTGGC | 2.03 |
| Tnfα | NM 008491.1 | ATGGGCTACAGGCTTGTCACTC | CTCTTCTGCCTGCTGCACTTTG | 2.25 |
18s: 18s ribosomal RNA, Cxcl-2: chemokine-ligand-2, Il-6: Interleukin-6, Kim-1: Kidney injury molecule-1, Mcp-1: monocyte chemoattractant protein-1, Ngal: neutrophile gelatinase-associated lipocalin, Tnfα: tumor necrosis factor.