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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Immunol Rev. 2016 Mar;270(1):152–164. doi: 10.1111/imr.12390

Figure 5. T cell activation of SS1P and LMB-T20.

Figure 5

Stimulation of PBMC from 10 donors with LMB-T20 and SS1P show a decrease in T cell activation. PBMC from 10 naïve donors were stimulated with either SS1P or LMB-T20. After 14 days of in vitro expansion cells that were re-stimulated with either 111 peptides spanning the sequence of PE38 or 76 peptides spanning the sequence of LMB-T20. T cell activation was detected using IL-2 ELISpot. All positive peptides were assayed twice for each PBMC sample and each assay was run in triplicate. Response strength is shown in the Spot Forming cell/million cells (SFC/1E6) ladder on the right. Black stars represent peptides that were deleted in LMB-T20 and red stars represent peptides that are different from wild type. This figure to be published in Mazor et al., Mol. Cancer Res., In press (75).