| Name of the buffer | Reagent | Concentration (mM) | Amount | Comments/Description |
| Gey’s solution (pH: 7.3~7.4) | NaCl | 130 | 7.6 g | sterile filtration |
| 1,000 ml | KCl | 4.9 | 0.37 g | |
| CaCl2·2H2O | 1.5 | 0.22 g | ||
| MgSO4·2H2O | 0.3 | 0.0739 g | ||
| MgCl2·6H2O | 11 | 2.24 g | ||
| KH2PO4 | 0.23 | 0.0313 g | ||
| Na2HPO4·7H2O | 0.8 | 0.2145 g | ||
| Glucose·H2O | 5 | 0.9909 g | ||
| HEPES | 25 | 5.96 g | ||
| NaHCO3 | 22 | 1.85 g | ||
| ACSF (pH: 7.3~7.4) | NaCl | 110 | 6.428 g | sterile filtration |
| 1,000 ml | KCl | 2.5 | 0.1865 g | |
| CaCl2·2H2O | 2.5 | 0.368 g | ||
| MgSO4·2H2O | 1.5 | 0.370 g | ||
| KH2PO4 | 1.24 | 0.169 g | ||
| Glucose·H2O | 10 | 1.9817 g | ||
| NaHCO3 | 27.4 | 2.3 g | ||
| 1x Hypotonic buffer (pH: 7.9) | HEPES | 10 | 0.23 g | |
| 100 ml | MgCl2·6H2O | 1.5 | 0.0304 g | |
| KCl | 10 | 0.07455 g |
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