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. 2016 Feb 18;11(2):e0149391. doi: 10.1371/journal.pone.0149391

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© 2016 Laura et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 8 — Cells grown on glass coverslips were fixed, permeabilized and double-labeled with polyclonal MPO antibodies and the indicated organelle marker antibody. Mounted coverslips were imaged with a 63X objective on a Zeiss LSM 710 confocal microscope. (A) T47D-C316A cells labeled with antibodies against MPO and Lamp1 and shows that the C316A mutant accumulates normally within lysosomes. (B) T47D-C319S cells labeled with antibodies against MPO (all panels) and Lamp1 (top panel), the ER marker KDEL (second panel), the trans-Golgi marker RCAS1 (third panel) and the cis-Golgi marker GM130 (fourth panel). Distinct localizations of the C319S mutant to both the ER (arrow) and Golgi (arrowhead) are indicated on the second panel. (C) T47D-R569W cells labeled with antibodies against MPO and the ER marker protein disulfide isomerase (PDI).