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. 2016 Feb 18;11(2):e0149667. doi: 10.1371/journal.pone.0149667

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© 2016 Yasuga et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — (a) Definition of the binary system based on the presence of DNA in an aqueous droplet coated with a lipid monolayer. (b) A schematic view of the bilayer lipid membrane (BLM) formed via DCM. (c) The system for DNA transfer among droplets by DNA translocation through αHL nanopores. (d–f) Electrical detection of DNA translocation and determination of the presence of DNA constructs in the droplet. (d) Output 1 (translocation): A single-stranded DNA (ssDNA) is translocated through an αHL nanopore with a short current blockade. (e) Output 0 (blocking): A double-stranded DNA (dsDNA) is not translocated through the αHL nanopore owing to its larger diameter, inducing a long current blockade. (f) Output 0 (No DNA strand): No DNA strand is present for translocation, and thus no current blockade is generated.