Table 2. Stability ranking of the tested genes evaluated with four statistical methods.
Ranking for OP Group | Delta-CT(Delta-CT Value) | geNorm (M Value) | BestKeeper (CV ± SD) | NormFinder (Stability Value) |
1 | mobk13(0.82) | lsm12b/actb2(0.376) | mobk13(2.22±0.43) | mobk13(0.162) |
2 | lsm12b(0.86) | lsm12b/actb2(0.376) | lsm12b(2.26±0.44) | lsm12b(0.384) |
3 | actb2(0.89) | mobk13(0.490) | actb2(2.85±0.52) | actb2(0.410) |
4 | actb1(1.01) | actb1(0.711) | actb1(5.47±0.78) | actb1(0.692) |
5 | C1H16orf72(1.18) | C1H16orf72(0.817) | C1H16orf72(5.79±0.91) | C1H16orf72(1.027) |
6 | ssr2(1.51) | ssr2(1.047) | ssr2(4.12±0.98) | ssr2(1.413) |
Ranking for RP Group | Delta-CT (Delta-CT Value) | geNorm (M Value) | BestKeeper (CV ± SD) | NormFinder (Stability Value) |
1 | mobk13(0.58) | ssr2/lsm12b(0.449) | ssr2(1.86±0.36) | mobk13(0.221) |
2 | C1H16orf72(0.64) | Ssr2/lsm12b(0.449) | mobk13(1.77±0.37) | C1H16orf72(0.379) |
3 | ssr2(0.67) | mobk13(0.470) | C1H16orf72(2.10±0.44) | ssr2(0.479) |
4 | lsm12b(0.69) | C1H16orf72(0.531) | lsm12b(2.35±0.49) | lsm12b(0.504) |
5 | actb1(0.80) | actb1(0.642) | actb1(3.55±0.59) | actb1(0.646) |
6 | actb2(0.81) | actb2(0.698) | actb2(4.91±0.79) | actb2(0.675) |