Fig 6. Dhp1-mediated silencing occurs independently of RNAi and the exosome.

(A-B) qRT-PCR analysis of silenced repeat regions in the centromere (A) and mating type locus (B) shows negative genetic interactions between dhp1-1 and ago1Δ or rrp6Δ. (C-D) H3K9me₂ ChIPs show the enrichments of H3K9me mark at indicated strains. Relative enrichments of H3K9me₂ at indicated genomic regions were normalized to leu1⁺. * p ≤ 0.05 as determined by Student’s t test comparing the indicated sample values with the wt values for qRT-PCR and qChIP. Significance between the single mutants and double mutants is indicated by horizontal lines linking the compared samples.