Table 2. Substrate specificity of purified asparaginase from E. cloacae.
| Substrate | Concentration (mM) | Relative activity (%)a |
|---|---|---|
| L-Asparagine | 10 | 100±1.9 |
| D-Asparagine | 10 | 2±0.4 |
| DL-Asparagine | 10 | 4±0.3 |
| L-Glutamine | 10 | N.D. |
| D-Glutamine | 10 | N.D. |
| L-Glutamic acid | 10 | N.D. |
| DL-Aspartic acid | 10 | 1.3±0.4 |
| L-Histidine | 10 | 1.1±0.3 |
| L-Ornithine | 10 | 1.2±0.2 |
| BOC-L-Asparagine | 10 | N.D. |
| N-α-Acetyl-L-Asparagine | 10 | N.D. |
| Urea | 10 | N.D. |
| Acrylamide | 10 | N.D. |
N.D. = Not detected
aRelative activity shown in the table. Each value represents the mean ±SD for three determinations.