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. 2016 Feb 18;11(2):e0149272. doi: 10.1371/journal.pone.0149272

Fig 8. Inhibition of Caveolae endocytosis blocks upregulation of ICAM-1 expression.

Fig 8

MLECs were pretreated with either 60 μM Dynasore or 0.1% DMSO for 30 min prior to incubation with MPs (Approx 40,000 MPs/mL) for the time points indicated (0, 0.5, 1, 2, 24 hrs). Cells were then harvested, separated with SDS-PAGE and blotted for ICAM-1 and β-Actin. MP-induced upregulation of ICAM-1 protein expression was significantly inhibited in cell treated with the dynamin-2 inhibitor dynasore. (Avg ± SEM two-tailed t-test, n = 4 each condition, *p<0.05).