Fig. 3.
Endogenous pDC recruitment to the SC following pDC transfer is mandatory for EAE protection. (A, B) EAE was induced in WT mice and BM-derived, MOG35-55-loaded, WT pDCs (EAE + pDC) or cDCs (EAE + cDC) were transferred or not (EAE) into mice during disease acute phase (arrow). (A) Clinical scores were followed daily. (B) Total pDC frequencies were analysed in SC of EAE mice four days after cell transfer. (C, D) EAE was induced in BDCA2-DTR:WT BM chimeric mice and BM-derived, MOG35-55-loaded, CD45.1 WT pDCs were transferred into EAE mice during EAE acute phase (D, red arrow, day 11). When indicated, mice were also injected with DT (D, black arrows, day 11 and day 14 after EAE induction). (C) Endogenous (gated on CD45.2 cells) and exogenous (gated on CD45.1 cells) pDC numbers (left) and total pDC frequencies (right) were analysed in SC of EAE mice four days after pDC transfer. (D) Clinical scores were followed daily. (A–D) Data are representative of at least 2 independent experiments with 8 mice per group. Data represent mean ± SEM. (A and D) 2-way ANOVA with Bonferroni post hoc test; (B, C right panel) 1-way ANOVA with Bonferroni post hoc test *P < 0.05; **P < 0.01; ***P < 0.001.