Fig. 4.
Chemerin-CMKLR1 axis mediates pDC recruitment to SC. EAE was induced or not in WT mice and BM-derived, MOG35-55-loaded, pDCs were transferred (EAE + pDC) or not (EAE) into mice during EAE acute phase. (A) Chemerin mRNA levels were analysed in SC of naïve and EAE mice day 12 after immunization. (B, C) CMKLR1 expression levels by cDCs and pDCs (B) in spleens one day after pDC transfer and (C) in SC one and four days after pDC transfer. (B) Graphs show the MFI and (C) the frequency of expressing cells. (D) EAE was induced in WT mice and WT BM-pDCs were transferred or not into mice during EAE acute phase. α-NETA was injected i.p. or not one day following pDC transfer for 3 consecutive days. Frequency of total pDCs was analysed in SC 4 days after pDC transfer. (A–D) Data are representative of 2 independent experiments with 4 mice per group each. Data represent mean ± SEM.(A–C) Standard two tailed Student's t test; (D) 1-way ANOVA with Bonferroni post hoc test. *P < 0.05; **P < 0.01; ***P < 0.001.