Table 1.
Early-onset seizures and related measures in aging and adult mice
| Aging mice | Adult mice | p value | |
|---|---|---|---|
| Animals with CS following MCAO | |||
| pMCAO | 8/13 (62%) | 11/17 (65%) | 1.0 |
| rMCAO90min | 12/19 (63%) | 11/15 (73%) | 0.715 |
| rMCAO45min | 15/27 (56%) | 3/6 (50%) | 1.0 |
| CS latencies (min) | 30.1±4.6 (n=27) | 45.6±9.9 (n=24) | 0.307 |
| Proportion of animals with CS onset during overnight video monitoring | 8/35 | 1/25 | 0.067 |
| NCS latencies (min) | 24.4±3.7 (n=13) | 17.8±5.1 (n=5) | 0.095 |
| NCS durations (sec) | 35.6±4.5 (n=14) | 42.9±4.3 (n=5) | 0.968 |
| Ipsilateral EEG changes in animals with CS (% of baseline) | |||
| 1 hr post MCAO | 41.4±5.4 (n=27) | 67.4±6.6 (n=11) | 0.010 |
| 24 hrs post MCAO | 57.8±16.5 (n=5) | 80.9±6.0 (n=8) | 0.391 |
| Weakly stained regions in animals with CS (% of ipsilateral hemisphere) | |||
| bregma 1.9 mm | 35.1±14.5 (n=3) | 36.7±7.1% (n=5) | 0.911 |
| bregma 1.2 mm | 18.2±7.4 (n=7) | 44.5±3.8 (n=5) | 0.048 |
| bregma 0.5 mm | 27.2±12.5 (n=6) | 52.9±2.8 (n=5) | 0.106 |
| bregma 0.2 mm | 24.7±12.9 (n=6) | 48.2±9.6 (n=6) | 0.176 |
| bregma −1.1 mm | 20.7±9.8 (n=4) | 45.8±8.1 (n=5) | 0.086 |
| bregma −1.5 mm | 28.9±11.1 (n=3) | 39.1±9.5 (n=5) | 0.525 |
| bregma −2.4 mm | 37.1 (n=1) | 36.2±10.9 (n=4) | |
| bregma −3.2 mm | 14.2 (n=1) | ||
| Area ratios of ipsilateral/contralateral hemisphere in animals with CS (%) | |||
| bregma 1.9 mm | 118.1±5.2 (n=13) | 114.6±3.2 (n=16) | 0.948 |
| bregma 1.2 mm | 116.1±2.1 (n=13) | 117.3±3.6 (n=17) | 0.801 |
| bregma 0.5 mm | 112.0±2.4 (n=11) | 117.1±3.3 (n=16) | 0.271 |
| bregma 0.2 mm | 113.0±3.7 (n=10) | 113.6±2.7 (n=16) | 0.890 |
| bregma −1.1 mm | 110.3±2.3 (n=11) | 111.7±2.3 (n=16) | 0.677 |
| bregma −1.5 mm | 109.3±1.7 (n=12) | 110.0±1.5 (n=15) | 0.791 |
| bregma −2.4 mm | 110.23.0 (n=13) | 109.7±2.7 (n=15) | 0.903 |
| bregma −3.2 mm | 107.5±3.3 (n=10) | 107.5±3.3 (n=12) | 0.167 |
| Acute mortality in animals received post-MCAO anticonvulsive treatment | |||
| pMCAO | 8/8 (100%) | 11/11 (100%) | 1.0 |
| rMCAO90min | 12/12 (100%) | 7/11 (64%) | 0.037 |
| rMCAO45min | 12/15 (80%) | 1/3 (33%) | 0.621 |
Numbers of animals examined are indicated in parentheses. Rate comparison was done using a Chi-square or Fisher exact test. Other comparisons were done using a Student’s t-test or Mann-Whitney Rank Sum Test. Two-tailed p values are presented, and bold font denotes where p≤0.05. For post-MCAO anticonvulsive treatment, intra-peritoneal injections of lorazepam (1.5mg/kg) and fosphenytoin (30mg/kg) were applied to individual animals after two observed CS events.