Figure 2. The eIF4F and eIF4F^H translation machineries govern O₂-dependent translatome remodeling.

(A) Global translation rates of U87MG transiently transfected with siRNA against the indicated proteins and non-silencing (NS) control siRNA were measured by puromycin incorporation. Loading was performed on an equal cell basis. %puro inc., percent puromycin incorporation. Immunoblots of silenced proteins are shown. β-actin was used as a loading control. (B) Schematic of eIF4F^H and eIF4F. (C) Immunoblots of eIF4E, eIF4E2, and HIF-2α endogenous IPs in normoxic and hypoxic U87MG. WCL, 5% whole cell lysate. (D) Polysome profiles and (E) pSILAC analysis of hypoxic U87MG stably expressing eIF4E2-specific or non-silencing (NS) control shRNA.