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. Author manuscript; available in PMC: 2017 Feb 16.
Published in final edited form as: Cell Rep. 2016 Feb 4;14(6):1448–1461. doi: 10.1016/j.celrep.2016.01.034

Figure 3. The N-terminal domain of AGO2 interacts with RAS.

Figure 3

(A) Schematic summary of FLAG tagged AGO2 deletion and mutant constructs used for RAS co-IP analyses (B) Expression of FLAG tagged N-terminal, PAZ, or PIWI domains of AGO2 in HEK293 cells (left panel), followed by RAS IP (right panel). Immunoblot analysis shows that deletion of (1-226aa) N terminal domain in AGO2 abrogates RAS interaction. (C) Expression of indicated AGO2 N terminal point mutant constructs within the wedge domain (50-139aa) in HEK293 cells, followed by RAS co-IP analysis. See also Figure S3.