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. Author manuscript; available in PMC: 2016 Mar 9.
Published in final edited form as: Cell Rep. 2016 Feb 4;14(6):1330–1338. doi: 10.1016/j.celrep.2016.01.023

Figure 2. Ligand-induced interactions between SERKs and HAE/HSL2.

Figure 2

(A, B) IDA-induced association of SERKs with HAE (A) and HSL2 (B) in Arabidopsis protoplasts. SERK-FLAG and HAE/HSL2-HA proteins were co-expressed with or without IDA expression in protoplasts, immunoprecipitated with α-FLAG antibody (IP: α-FLAG), and immunoblotted with α-HA (IB: α-HA) or α-FLAG antibody (IB: α-FLAG) (top two panels). The protein inputs are shown with immunoblotting before immunoprecipitation (bottom two panels). (C) IDA induces the association between BAK1 and HAE/HSL2 in Nicotiana benthamiana. (D) IDA-induced interaction of BAK1 and HAE/HSL2 in a BiFC assay. BAK1-nYFP and HAE/HSL2-cYFP were transiently co-expressed with or without IDA expression in the leaves of N. benthamiana. YFP signals were observed in the epidermal cells using a confocal microscopy.