Figure 2.

ZDF fa/fa or lean liver mitochondrial H₂O₂ production. Liver mitochondria were isolated as described in Materials and Methods. H₂O₂ production rate was determined at 30°C by incubating mitochondria (0.2 mg mL ⁻¹) in a respiration buffer (see Materials and Methods) with 6 IU horseradish peroxidase and 1 μmol L⁻¹ Amplex Red. Measurements were carried out with various substrates: 5 mmol L⁻¹ glutamate/2.5 mmol L⁻¹ malate (A), 5 mmol L⁻¹ succinate (B), or Palmitoyl‐carinitine (C) and after sequential addition of rotenone (Rot) and antimycin A (+Rot+AA). Values are expressed as means ± SEM (n = 7 rats in each group). a ≠ b with P < 0.05 on each graph.