Table 4.
Content of mitochondrial respiratory chain complex in ZDF rats liver mitochondria
| 11 weeks | 14 weeks | |||
|---|---|---|---|---|
| lean | fa/fa | lean | fa/fa | |
| Complex I | 1.00 ± 0.10 | 0.91 ± 0.07 | 1.02 ± 0.12 | 1.04 ± 0.22 |
| Complex II | 1.00 ± 0.07 | 1.03 ± 0.06 | 1.09 ± 0.13 | 1.06 ± 0.23 |
| Complex III | 1.00 ± 0.03 | 1.12 ± 0.29 | 1.07 ± 0.17 | 1.32 ± 0.24 |
| Complex IV | 1.00 ± 0.09 | 0.97 ± 0.18 | 1.02 ± 0.13 | 1.16 ± 0.21 |
| ATP synthase | 1.00 ± 0.11 | 1.04 ± 0.06 | 1.08 ± 0.14 | 1.02 ± 0.12 |
| ANT2 | 1.00 ± 0.03a | 0.78 ± 0.04b | 0.94 ± 0.06a | 0.61 ± 0.04b |
| UCP2 | 1.00 ± 0.05a | 1.14 ± 0.06a | 0.66 ± 0.07b | 0.65 ± 0.03b |
Protein expressions of complex I‐IV, ATP synthase, ANT2, UCP2 were determined by Western blotting. Data were normalized to the expression of VDAC‐1 and are presented as mean ± SEM (n = 7). a ≠ b with P < 0.05 on each line. a and b are statistically different.