Table 5.
Effect of CsA on the Ca2+ retention capacity of isolated ZDF rats mitochondria
| 11 weeks | 14 weeks | |||
|---|---|---|---|---|
| lean | fa/fa | lean | fa/fa | |
| G/M (μmolCa2+ mg Prot−1) | 235 ± 13 | 275 ± 18a | 233 ± 14 | 219 ± 19b |
| G/M + CsA (μmolCa2+ mg Prot−1) | 515 ± 19a | 445 ± 28 | 525 ± 35a | 417 ± 35b |
| CsA‐G/M (μmolCa2+ mg Prot−1) | 280 ± 10a | 170 ± 17b | 293 ± 25a | 198 ± 20b |
| Succ (μmolCa2+ mg Prot−1) | 225 ± 18a | 290 ± 6b | 210 ± 15a | 250 ± 20 |
| Succ + CsA (μmolCa2+ mg Prot−1) | 380 ± 12 | 400 ± 8a | 348 ± 20b | 363 ± 15 |
| CsA‐Succ (μmolCa2+ mg Prot−1) | 155 ± 18a | 110 ± 6b | 138 ± 18 | 112 ± 12b |
The CRC were determined on liver mitochondria extracted from ZDF fa/fa or lean rats at the age of 11 weeks or 14 weeks using glutamate/malate (G/M) or succinate (Succ) as substrates and either in the presence or not of CsA. Effect of CsA was calculated as the difference between CRC in the presence or not of 1 μmol L−1 CsA. Data are presented as mean ± SEM (n = 7). a ≠ b with P < 0.05 on each line. a and b are statistically different.