Table 3.

Intra-molecular binding constants (see Fig. 5 for definition) and apparent equilibrium dissociation constants determined for ITP complexes of tSH2, tSH2_PM and tSH2_FX

Complex	3-state model		10-state model
	K′N (10−3)	KApp (μM)a	K′N (10−3)	K′C (10−3)b	KApp (μM)c
tSH2 / ITP	<< 5	<< 0.3	<< 4	<< 0.7	<< 0.3
tSH2PM / ITP	38 ± 8	3.2 ± 0.7	32 ± 10	4.4 ± 1.6	2.7 ± 0.8
tSH2FX / ITP	120 ± 20	6.1 ± 1.3	100 ± 10	11 ± 3	5.5 ± 1.1

^aK^App = K_CN × K′_N.

^bK′_C = K_CN × K′_N / K_NC.

^cK^App = K_CN × K′_N = K_NC × K′_C.

See Table 2 for the values of K_CN and K_NC determined from IHP complexes (line-shape analysis).

Data are shown as mean ± SD or detection limit of fitted values from three independent experiments.