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Apparent VL molecular weights and ligand binding derived from analytical ultracentrifugation.
| Speed (rpm) | Concentration of Mcg (μM) | Ligand | Concentration of ligand (μM) | Apparent molecular weight (Da) | Oligomer state |
|---|---|---|---|---|---|
| 22,000 | 80 | methylene blue | 100 | 27,342 ± 268* | Dimer + multimer |
| 150 | 26,507 ± 225* | Dimer + multimer | |||
| 35,000 | 80 | methylene blue | 100 | 23,609 ± 212* | Dimer |
| 150 | 23,397 ± 152* | Dimer | |||
| 22,000 | 200 | sulfasalazine | 300 | 21,989 ± 571* | Dimer |
| 500 | 21,821 ± 345* | Dimer |
*Standard error.
