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. 2015 Dec 19;4:e12997. doi: 10.7554/eLife.12997

Figure 3. Inhibition of activin A alleviates the impairment of adipogenesis induced by senescent progenitors.

Figure 3.

CM was collected from control (CON) and senescent (SEN) fat progenitors. Pooled human progenitors were treated with a 50:50 mixture of CM:DM in the presence of DMSO or 5μM SB431542 (SB431542). (a) Representative images are shown of differentiated cells at day 15. (b) RNA was collected 7 days after differentiation and real-time PCR was performed. Pooled human progenitors were treated with a 50:50 mixture of CM:DM in the presence or absence of 1μg/ml activin A neutralizing antibody (Activin A AB). (c) Representative images are shown of differentiated cells at day 15. (d) RNA was collected 7 days after differentiation and real-time PCR was performed. Results are shown as fold change relative to the SEN group. Results were obtained using CM from 5 strains of human primary cells from different subjects and expressed as mean ± s.e.m. *p<0.05. Two-tailed Student's t tests were used to determine statistical significance.

DOI: http://dx.doi.org/10.7554/eLife.12997.008

Figure 3—source data 1. Inhibition of activin A alleviates the impairment of adipogenesis induced by senescent progenitors.
DOI: http://dx.doi.org/10.7554/eLife.12997.009
elife-12997-fig3-data1.xlsx (13.3KB, xlsx)
DOI: 10.7554/eLife.12997.009