Figure 5. JAK inhibition suppresses activin A production by senescent fat progenitors and partially rescues adipogenesis.

Senescent human progenitors were treated with DMSO (SEN) or 0.6 μM JAK inhibitor 1 (SEN+JAKi) for 72 hours. (a) RNA was collected from control (CON), SEN, and SEN+JAKi progenitors and real-time PCR was performed. Results (N=7) are expressed as mean ± s.e.m. *p<0.05. (b) CM was collected and activin A protein was assayed by ELISA. Results (N=6) are expressed as mean ± s.e.m. *p<0.05. (c) Representative images are shown of differentiating cells at day 10. (d) RNA was collected 10 days after initiation of differentiation and real-time PCR was performed. Results are shown as fold change relative to the SEN group. Results were obtained using CM from 7 strains of human primary progenitors from different subjects and expressed as mean ± s.e.m. *p<0.05. Two-tailed Student's t tests were used to determine statistical significance.

DOI: http://dx.doi.org/10.7554/eLife.12997.014

Figure 5—figure supplement 1. Impaired adipogenesis due to effects of senescent cells is partially rescued by JAK inhibition.

(a) CM was collected from non-senescent (CON) and senescent (SEN) fat progenitor cultures. JAK inhibitor 1(0.6 µM) was directly added into CON (CON+JAKi) and SEN (SEN+JAKi) CM. Pooled human fat progenitors were treated with 50:50% CM:DM for 10 days. Gene expression was analyzed by real-time PCR. Results were obtained using CM from 3 strains of human primary fat progenitors and expressed as mean ± s.e.m. (b) Rat fat progenitors were isolated from 3 and 30-month old rats. These cells were differentiated in presence of DMSO or 0.6µM JAK inhibitor 1. Representative pictures were shown 48 hours after initiation of differentiation. (c) Gene expression was analyzed by real-time PCR in fat progenitors from 30-month old rats. Results (N=4) are expressed as mean ± s.e.m. *p<0.05. Two-tailed Student's t tests were used to determine statistical significance.

DOI: http://dx.doi.org/10.7554/eLife.12997.016