Figure 7. JAK inhibition increases adipogenic markers in adipose tissue and decreases circulating free fatty acids in aged mice.

Twenty-two-month old male mice were treated with vehicle (CON) or ruxolitinib (INCB) for 8 weeks. (a) Weights of different fat depots and liver are shown as percent of whole body weight. Results (N=9) are expressed as mean ± s.e.m. *p<0.05. (b) RNA from WAT was isolated and real-time PCR was performed. Results (N=8) are expressed as mean ± s.e.m. *p<0.05. (c) Plasma activin A protein levels were assayed by ELISA in parallel from 8 six-month-old male mice (Young). Results (N=15 for CON and INCB, N=8 for Young) are expressed as mean ± s.e.m. *p<0.05. Plasma TG (d) and FA (e) levels are expressed as mean ± s.e.m. (N=8). *p<0.05. (f) Hepatic TG/protein levels are expressed as mean ± s.e.m. (N=11). (g) Total hepatic TG levels are expressed as mean ± s.e.m. (N=11). Two-tailed Student's t tests were used to determine statistical significance.

DOI: http://dx.doi.org/10.7554/eLife.12997.021

Figure 7—figure supplement 1. JAK inhibition in aged mice suppressed activin A expression in primary fat progenitors.

Twenty-two-month old male mice were treated with vehicle (CON) or ruxolitinib (INCB) for 8 weeks. Fat progenitors were isolated from WAT and gene expression was analyzed by real-time PCR. Some progenitors were pooled from several mice within the same treatment group due to limited yield of cells. Results (N=5 pools, each from different sets of mice) are expressed as mean ± s.e.m. *p<0.05. Two-tailed Student's t tests were used to determine statistical significance.

DOI: http://dx.doi.org/10.7554/eLife.12997.023