Table 1.

Experimentally determined (Parent inactive mutant, suppressor) pairs for CcdB occur at both spatially proximal and distal residues.

DOI: http://dx.doi.org/10.7554/eLife.09532.008

Parent inactive mutant (X)	Suppressor Mutant (Y)	ASA (%)	Depth (Å)	RankScorea	Shortest distanceb (Å)	Centroid-centroid distancec (Å)
V5Fd		0	6.8	54
	L36Md,e	0	7.3	83	3.9	5.5
	A81Gd,e	32.4	3.9	26	2.8	4.2
V18W		0	9.3	59
	M63Te	0.1	8.1	46	4.2	5.8
	I90Ve	0.1	7.4	60	4.8	5.9
V20F		0	8.6	60
	E11Rf	112.7	3.4	1	18.4	22.4
	E11Kf	112.7	3.4	1	18.4	22.4
L36A		0	7.3	83
	M63Le	0.1	8.1	46	3.8	6.4
	R10Gf	76.6	3.6	1	11.6	18.1
	E11Pf	112.7	3.4	1	11.7	16.8
L83S		1.5	5.8	42
	V54Le	0.4	5.7	28	3.9	4.5

^aRankScore for residues X or Y estimated from phenotypic screening of single-site saturation mutagenesis library of CcdB and deep sequencing (Adkar et al., 2012).

^bShortest distance between residues X and Y.

^cDistance between side chain centroids of residues X and Y.

^dThe suppressors (Y) were identified as a triple mutant with the parent inactive mutant (X) V5F, that is V5F/L36M/A81G.

^eSuppressor residues spatially proximal to parent inactive mutant.

^fSuppressor residues distal from the parent inactive mutant.

Heavy atoms of the residues are considered for calculating distances using the crystal structure of CcdB, PDB id 3VUB (Loris et al., 1999).