Table 2.
List of Antibodies used in this study. Full description of the antibodies and the amounts or concentrations used in this study for WB, ChIP or IF.
| Amount/dilution | |||||||
|---|---|---|---|---|---|---|---|
| Antibody | Raised in (isotype) |
Clone | Stock | WB | ChIP | IF | Source |
| S5p | Mouse (IgG) | CTD4H8 (MMS-128P) | 1 mg/ml | 1/200,000 | 10 µl (10 µg) | 1/3000 | Covance |
| S7p | Rat (IgG) | 4E12 | - | 1/10 | - | - | Kind gift from Dirk Eick |
| S2p | Mouse (IgM) | H5 (MMS-129R) |
1–3 mg/ml | 1/500 | - | - | Covance |
| Unphospho-S2 | Mouse (IgG) | 8WG16 (MMS-126R) |
1–3 mg/ml | 1/200 | - | - | Covance |
| N-terminus (Total RPB1) |
Rabbit (IgG) | H224 (sc-9001x) |
200 µg/ml | 1/200 | - | - | Santa Cruz Biotechnology |
| K7me1 | Mouse (IgG) | CMA611 | 10 mg/ml | 1/1000 | 5 µl (50 µg) |
1/200 | This study |
| K7me2 | Mouse (IgG) | CMA612 | 10 mg/ml | 1/1000 | 5 µl (50 µg) |
1/200 | This study |
| H3K9ac | Rabbit serum | 39585 | - | 1/1000 | - | - | Active Motif |
| Lamin B | Goat (IgG) | C-20 Sc-6216 | 200 µg/ml | 1/500 | - | Santa Cruz Biotechnology |
|
| α-tubulin | Mouse (IgG) | T6074 | 2 mg/ml | 1/10,000 | - | - | Sigma |
| GFP | Rabbit (IgG) | A11122 | 2 mg/ml | 1/1000 | - | - | Life Technologies |
| Digoxigenin | Mouse (IgG) | 200–002-156 | 1.2 mg/ml | - | 10 µl (12 µg) | - | Jackson ImmunoResearch |
ChIP, chromatin immunoprecipitation; IF, immunofluorescence; WB, western blotting.