Figure 8. Cohesin is removed by a WAPL-1-independent mechanism in late diakinesis oocytes.
Projections of diakinesis oocytes from worms expressing SMC-1::GFP (tagged by CRISPR) stained with anti-GFP antibodies and DAPI, and imaged with a Delta Vision system (A) or a structured illumination microscope (B). WT oocytes accumulate a large amount of nuclear soluble SMC-1::GFP that is lacking in wapl-1 mutant oocytes, but a reduction in chromosome-associated SMC-1::GFP occurs in -1 oocytes of both WT and wapl-1 mutants. -1 and -2 oocytes shown for each genotype were part of the same germ line and were acquired on the same image. Scale bars = 5 µm. (C) Table showing analysis of reduction in chromosome-associated SMC-1::GFP staining in late diakinesis oocytes of WT and wapl-1 mutant worms.
Figure 8—figure supplement 1. WAPL-1-independent reduction in SMC-1::GFP staining during late meiotic prophase.
Projection of diakinesis oocytes from wapl-1 mutants expressing SMC-1::GFP and stained with anti-GFP antibodies and DAPI. The numbers at the top of the panel indicate the position of oocytes within the germ line (-1 oocyte is about to be ovulated and enter the first meiotic division). Note the decrease in chromosome-associated SMC-1::GFP signal between oocytes -3 and -2. Scale bar = 5 µm.