Table 1.
Primers used for the amplification of the 10 exons of the human Tektin-t gene
| Exon | Primer | Primer sequence | Size (bp) | PCR product size (bp) |
|---|---|---|---|---|
| 1 | tektin-t 1 F | 5′ AGTGAAAGCGCTGCCCTGAT 3′ | 20 | 384 |
| tektin-t 1R | 5′ GGAGAGCCCCGTGGGAAGAG 3′ | 20 | ||
| 2-3 | tektin-t 2 F | 5′ CAGAGCAAAATGGACAGGAA 3′ | 20 | 661 |
| tektin-t 3R | 5′ TAGCGCTTGTCCCCACTTTA 3′ | 20 | ||
| 4 | tektin-t 4 F | 5′ GCCATCACTCCCAGCTAA 3′ | 18 | 488 |
| tektin-t 4R | 5′ GCCAAAATGTAGCACGAGAG 3′ | 20 | ||
| 5-7 | tektin-t 5 F | 5′ CCTGTGGTGTCCCTTTAGTA 3′ | 20 | 894 |
| tektin-t 7 F | 5′ TCAGGCTCAGGAGCTTTGTG 3′ | 20 | ||
| 8-10 | tektin-t 8 F | 5′ ACTTCCTGCTGTGGGATGAG 3′ | 20 | 936 |
| tektin-t 10R | 5′ CCTCTGCATTTATTCCATTC 3′ | 20 |
F forward primer, R reverse primer