TABLE 4.

Saa3 gene expression measured by qPCR in unflushed tibia and Saa3 protein expression in serum

WT and Cox2 KO mice (3-month-old males) were infused with vehicle or PTH (40 μg/kg/days) for 12 days prior to obtaining serum and dissecting out tibiae. Values are mean ± S.E. from n mice.

Tissue	WT mice		Cox2 KO mice
	Vehicle	PTH	Vehicle	PTH
Tibia, mRNA (RQ)a	0.086 ± 0.01	4.11 ± 0.96b	0.07 ± 0.01	0.19 ± 0.08c
	n = 7	n = 7	n = 7	n = 7
Serum (μg/ml)	1/5 Detectable	5/5 Detectable	2/5 Detectable	1/4 Detectable
	0.13	1.28 ± 0.11	0.09, 0.10	0.15
	n = 5	n = 5	n = 5	n = 4

^a RQ, relative quantification values.

^b Significant effect of PTH, p ≤ 0.01.

^c Significant effect of genotype, p ≤ 0.01.