Skip to main content
. 2015 Dec 23;291(8):3918–3931. doi: 10.1074/jbc.M115.675413

FIGURE 4.

FIGURE 4.

Correlation between Ser-235 phosphorylation and HCV replication. Shown are representative (A) and summary (B) results of time course immunoblotting for NS5A in the HCV (J6/JFH-1)-infected Huh7.5.1 cells. C, a scatter plot of the wild type HCV reporter virus activity (Fig. 2, B and C) against Ser(P)-235/NS5A ratio (Fig. 4B) at the indicated hours after HCV infection. r, Pearson's correlation coefficient with p value in parentheses. D, time course immunoblotting for NS5A in the Huh7.5.1 cells transfected with the wild type subgenomic replicon (pSGR-JFH-1). E, immunoblotting for NS5A in the Huh7.5.1 cells transfected with the WT full-length (J6/JFH-1) and various serine-to-alanine mutant transcripts. F, immunoblotting for NS5A in the Huh7.5.1 cells transfected with the wild type subgenome (pSG-JFH-1) and various serine-to-alanine mutant transcripts. G and H, representative and summary results of the NS5A protein levels in the Huh7.5.1 cells transfected with the wild type and various alanine mutant NS5A constructs. Error bars, S.E.