Skip to main content
. 2015 Dec 23;291(8):3918–3931. doi: 10.1074/jbc.M115.675413

FIGURE 8.

FIGURE 8.

Phosphorylation of NS5A Ser-235 by casein kinase Iα. A and B, immunoblotting and confocal immunofluorescence staining for CKIα and NS5A in the HCV (J6/JFH-1)-infected Huh7.5.1 cells. C, in vitro kinase assay. The biotin-labeled synthetic NS5A-101 peptide was incubated with or without CKIα before Ser-235 phosphorylation (pS235) was assessed with dot blotting. The fluorescence-conjugated streptavidin was used to detect biotin for peptide loading control. The numbers indicate signal intensity. p < 0.05. Error bar, S.E.

HHS Vulnerability Disclosure