TABLE 1.
Phosphopeptides identified for the HCV non-structural protein NS5A
The Ascore is an estimate for false discovery rate of a phosphorylation site (boldface and underlined letters). An Ascore of >20 indicates a false discovery rate of <1%. Numbering was based on NS5A (genotype 2a, JFH-1 isolate, Uniprot accession number Q99IB8). MH+, peptide mass; Xcorr, cross-correlation score; ΔCn, Xcorr difference from the next hit; Ions, number of peptides matched.
| Peptide sequence | MH+ | Charge | Xcorr | ΔCn | Ions | Ascore |
||
|---|---|---|---|---|---|---|---|---|
| Ser-222 | Ser-235 | Ser-238 | ||||||
| GSPPSEASSSVSQLSAPSLR | 2,023.9 | 3 | 4.96 | 0.09 | 41/112 | 24.75 | ||
| GSPPSEASSSVSQLSAPSLR | 2,023.9 | 2 | 4.98 | 0.17 | 32/56 | 44.73 | ||
| GSPPSEASSSVSQLSAPSLR | 2,023.9 | 2 | 2.99 | 0.31 | 22/56 | 27.99 | ||
| GSPPSEASSSVSQLSAPSLR | 2,103.9 | 3 | 3.81 | 0.26 | 45/148 | 39.63 | 45.69 | |