FIGURE 5.

Transcriptional regulation of c-Maf by c-Jun and Etv5/ERM. A, distribution of c-Jun and Etv5/ERM factors and H3 K4me3 promoter marker along the mouse c-Maf locus in lens chromatin. The locations of the qChIP amplicons covering 11 kb (−5 kb/+6 kb) c-Maf locus is shown at top. c-Jun, Etv5/ERM, and H3K4me3 are presented in the middle and lower panel, respectively. Statistically significant enrichment of binding signals is indicated above the horizontal dotted line. The relative enrichments are shown as 1% of the input. B, diagram of firefly luciferase reporter constructs without promoter (pGL3-Basic), with the intact −494/+210 c-Maf promoter (WT), with the c-Maf promoter lacking the −272/-70 FRE region (ΔFRE), and with three copies of the FRE followed by a minimal E4TATA promoter (3XFRE). C, results of transient co-transfection/reporter assays. The firefly luciferase activities were normalized using Renilla luciferase as an internal control. The results shown are from two independent experiments with duplicates. The relative luciferase activities were calculated using the “WT reporter with pCMV6 cDNA vector” value set as 1.