FIGURE 6.

Spectral count comparison of the identified 103 proteins. a, 64 glycoproteins that were labeled by ST6Gal1 SEEL but not by ST3Gal1 SEEL. b, 28 glycoproteins that were labeled both by ST6Gal1 and ST3Gal1 SEEL. c, 11 glycoproteins that were labeled by ST3Gal1 but not by ST6Gal1. The spectral counts for specific glycoproteins tagged by SEEL generally exceeded those obtained by metabolic labeling. In addition, the identity of those glycoproteins only detected by SEEL is shown. Two independent biological experiments for SEEL and metabolic labeling in the undifferentiated cells were performed, with the same subset of major glycoproteins identified in both runs. A representative comparison is shown.