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. 2016 Jan 5;291(8):3982–3989. doi: 10.1074/jbc.M115.700369

FIGURE 7.

FIGURE 7.

Changes of N- and O-sialoglycoprotein level of representative sialoglycoproteins upon differentiation of HEL cells. Spectral count analysis for selected glycoproteins enriched and detected by SEEL with either ST6Gal1 or ST3Gal1. Control: HEL cells that were only treated by S-DIBO; ST6Gal1: HEL cells only labeled by SEEL with ST6Gal1; ST3Gal1: HEL only labeled by SEEL with ST3Gal1. U refers to undifferentiated HEL cells, and D refers to HEL cells differentiated to megakaryocytes following PMA stimulation. The results from a representative analysis are shown. All SEEL labeling were done with prior neuraminidase treatment followed by S-DIBO-biotin. The data shown are derived from a single proteomic analyses of undifferentiated and differentiated HEL cells labeled at the same time under identical conditions.