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. 2015 Dec 23;291(8):4048–4057. doi: 10.1074/jbc.M115.680298

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FIGURE 5. — Knockdown efficiency and specificity of human B7-H3 siRNA. A, knockdown efficiency of B7-H3. B7-H3-expressing THP-1 cells were transfected with human B7-H3-specific or control siRNA (100 μm of both siRNAs), and the surface expression of B7-H3 was analyzed at 48 h after transfection using flow cytometry. B, differential knockdown effect of human B7-H3 siRNA on 2Ig and 4Ig B7-H3 isoforms. B7-H3 expression in whole lysates was analyzed using immunoblotting at 48 h after siRNA transfection. Horizontal bars and error bars show the means ± S.E. **, p < 0.01 by paired t test in A. MFI, mean fluorescence intensity.