FIGURE 10.

HA enhanced the adhesion of CD4⁺ cells to tumor tissues. A, detection of F4/80⁺/CD206⁺ cells in dissociated KYSE-410 xenograft tumors. Gating was performed on living CD45⁺/CD11b⁺ cells. Bottom, fluorescent staining of HA (yellow), Mac2 (red), and CK-18 (green) in KYSE-410 xenograft tumors. Scale bar, 100 μm. B, adhesion of activated CD3⁺ cells (white) to xenograft tumor sections with or without prior hyaluronidase (Hyal) digestion. Nuclei were stained with DAPI (blue). C, number of CD3⁺ cells bound to xenograft tumor sections normalized to the area and depicted as -fold of control (n = 6). D, binding of activated CD4⁺ cells (white) to xenograft tumor sections with or without prior Hyal digestion. Nuclei were stained with DAPI (blue). E, number of CD4⁺ cells bound to xenograft tumor sections normalized to the area and depicted as -fold of control (n = 6). F, adhesion of activated CD8⁺ cells (white) to control xenograft tumor sections or to sections that were digested with Hyal. DAPI staining is shown in blue. G, number of bound CD8⁺ cells to xenograft tumor sections normalized to the area and depicted as -fold of control (n = 6). Data are presented as mean ± S.E. (error bars). *, p < 0.05.