FIGURE 6.

The KYSE-410 phenotype was not altered by increased HA synthesis in direct co-culture. A, B, C, and D, mRNA expression of mesenchymal markers fibronectin (FN1), vimentin (VIM), and SNAI1 and epithelial marker CDH1 in KYSE-410 cells after 96 h of monoculture or co-culture with either control SF or Has2 knockdown SF (n = 4). E, cell count of EpCAM⁺ KYSE-410 cells by flow cytometry after 3 days in monoculture and co-culture (n = 4). F, gene expression of the proliferation marker MKI67 in KYSE-410 cells in mono- and co-culture with either control SF or Has2 knockdown SF (n = 3). G, random migration of KYSE-410 cells in direct co-culture with control SF or Has2 knockdown SF (n = 3). Data are presented as mean ± S.E. (error bars).