FIGURE 7.

Has2 up-regulation in co-culture may modulate the myofibroblast phenotype. A, Western blotting analysis of αSMA expression in direct co-cultures of KYSE-410 cells and control SF or Has2 knockdown SF 48 h after seeding (n = 4). B, SF proliferation as measured by decreasing fluorescence of CFSE-labeled SF (n = 3). C, random migration speed of SF determined by time lapse microscopy in SF monocultures and direct co-cultures of KYSE-410 cells and control SF or Has2 knockdown SF (n = 3). Data are presented as mean ± S.E. (error bars). *, p < 0.05.