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. 2015 Dec 23;291(8):4091–4106. doi: 10.1074/jbc.M115.708909

FIGURE 8.

FIGURE 8.

Direct co-culture with KYSE-410 cells induced CCL5 and reduced CCL11 chemokine expression by fibroblasts. A, mRNA expression of murine chemokines Ccl2, Ccl5, Ccl7, Ccl11, Cxcl1, and Cxcl12 in SF after 48 h in monoculture or co-culture (n = 4). B, murine CCL5 protein concentration in cell culture supernatants of SF and KYSE-410 monoculture and direct co-cultures 48 h after seeding, determined by ELISA (n = 4). C, Ccl5 mRNA expression in control SF and Has2 knockdown SF after 48 h of co-culture with KYSE-410 cells (n = 4). D, Ccl5 mRNA expression in SF after treatment with 300 μm 4-MU or vehicle for 48 h (n = 4). E, expression of chemokine receptor CCR1 mRNA in KYSE-410 cells after 48 h in mono- and co-culture (n = 3). F, murine CCL11 protein concentration in supernatants of SF and KYSE-410 monocultures and direct co-cultures conditioned for 60 h (n = 4). G, Ccl11 mRNA expression in control SF and Has2 knockdown SF after 48 h in monoculture or co-culture (n = 4). H, Ccl11 mRNA expression in SF after treatment with 300 μm 4-MU or vehicle for 48 h (n = 4). Data are depicted as mean ± S.E. (error bars). *, p < 0.05; n.s., not significant; n.d., below the limit of detection.