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. 2015 Dec 23;291(8):4091–4106. doi: 10.1074/jbc.M115.708909

FIGURE 9.

FIGURE 9.

Has2 and Ccl5 mRNA expression were also induced, and Ccl11 mRNA expression was reduced in CAF in direct co-culture with KYSE-410 cells. A, Has2 mRNA expression in CAF after 24 h of direct co-culture with KYSE-410 cells (n = 10). B, Western blotting analysis of αSMA expression in direct co-cultures of KYSE-410 cells and CAF treated with 300 μm 4-MU or vehicle for 48 h (n = 3). C, Ccl5 mRNA expression in CAF after 48 h of treatment with 300 μm 4-MU or vehicle (n = 4). D, Ccl11 mRNA expression in CAF after 48 h of treatment with 300 μm 4-MU or vehicle (n = 4). E–H, mRNA expression of EMT markers in KYSE-410 cells co-cultured with CAF for 96 h and treated with 4-MU or vehicle for the last 72 h (n = 4). I, cell count of KYSE-410 as determined by EpCAM+ cells in flow cytometry after 3 days in culture (n = 5). J, average migration speed of KYSE-410 cells in mono- and co-culture with CAF (n = 3). Data depicted as mean ± S.E. (error bars). *, p < 0.05; n.s., not significant.