FIGURE 3.

Different bone marrow populations expanded during emergency granulopoiesis in wild-type versus Icsbp^−/− mice. Mice were injected with Alum to stimulate emergency granulopoiesis or saline as a steady-state control and sacrificed 2 weeks later. A, during steady-state granulopoiesis, populations of Gr1⁺, Ly6G⁺, Ly6C⁺Ly6G⁻, and c-kit⁺ bone marrow cells were greater in Icsbp^−/− versus WT mice, and, during emergency granulopoiesis, the CD34⁺ population was also greater in Icsbp^−/− mice. Bone marrow was evaluated by flow cytometry, and cell numbers were calculated. Statistically significant differences are indicated by *, **, ***, #, ##, ###, &, &&, &&&, and +. B, Ly6C⁺Ly6G⁺ and Ly6G⁺CD11b⁺ cells increased more in the bone marrow of WT mice in comparison with Icsbp^−/− mice 2 weeks after emergency granulopoiesis stimulation. The study was repeated three times, and representative histograms are shown. C, during emergency granulopoiesis, Icsbp^−/− mice had a relatively greater increase in CD34⁺ cell populations, but WT mice had a greater relative increase in granulocyte and monocyte populations. The data above were analyzed for percent change. Statistically significant differences are indicated by *, **, ***, #, ##, ###, &, and &&. D, sternal bone marrow from Alum-injected WT or Icsbp^−/− mice showed abundant mature granulocytes, but only Alum-injected Icsbp^−/− mice demonstrated pulmonary infiltration with granulocytes. Tissues were stained with H&E and examined by light microscopy at ×40 magnification. p < 0.02 was considered statistically significant.